Course: Analytical Instrumental Methods

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Course title Analytical Instrumental Methods
Course code KALCH/C023
Organizational form of instruction Lecture + Seminary
Level of course Bachelor
Year of study 3
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Lísa Miroslav, Ing. Ph.D.
  • Vytřas Karel, prof. Ing. DrSc.
  • Mikysek Tomáš, Ing. Ph.D.
  • Meloun Milan, prof. RNDr. DrSc.
  • Švancara Ivan, prof. Ing. Dr.
  • Metelka Radovan, Ing. Ph.D.
  • Ventura Karel, prof. Ing. CSc.
  • Eisner Aleš, Ing. Ph.D.
Course content
Potentiometry. Electrodes and cells. Glass pH-measuring electrode, experimental approach to measurement of pH. Ion-selective electrodes. Nikolsky-Eisenman equation. Electrodes with membranes. Experimental setups of potentiometric titration, evaluation of titration curves. Polarography/voltammetry. Basics of polarographic measurements. Mercury drop electrode and its properties. Sample preparation for polarographic measurement. Recent trends in voltammetry. Types of titration curves, their interpretation and applications. Electrogravimetry and coulometry. Conductometry and conductometric titrations. Basic approaches and instruments in optical emission spectrometry. Flame photometry. Atomic absorption spectrometry (AAS). Basic setup of atomic absorption spectrometer. Flame atomisation and eletrotermical atomisation. Calibration and applications of AAS. Molecular absorption spectrometry in UV/VIS range. Basic photometric quantities. Bouguert-Lambert-Beer law. Possibilities of identification of compounds. Quantitative analysis. Photometric (micro)titrations. Luminiscence spectrometry. Principles of infrared and Raman spectrometry. Nonspectrometric optical methods. Polarimetry, refractometry, interferometry. Nephelometry and turbidimetry. Extractions, extraction equilibria, definition of equilibrium constants. Extraction of solid compound by liquid and supercritical fluid. Extraction from liquid to liquid, extraction of neutral chelates, extraction of ionic associates. Solid phase extraction, extraction by gas. Chromatography. Basic principles, classification of chromatography according to the mechanism of separation, introduction of sample etc. Processing of chromatographic data. Qualitative and quantitative analysis. Gas chromatography. Liquid chromatography. Paper and thin-layer chromatography. Electromigration separation methods. Electrophoresis and isotachophoresis.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
Learning outcomes
The aim of the course is to extend knowledge of classical analytical methods with basic knowledge about instrumental analytical methods.
Students after graduation of the subject "Analytical instrumental methods" together with the subject C024 "Laboratory of analytical instrumental methods" are competent to execute the simpler procedures in analytical laboratories equipped with the basic accessories
Prerequisites
High school knowledge of analytical chemistry
KALCH/C024 and KALCH/C019

Assessment methods and criteria
Oral examination, Written examination

Current check of study - 2 written tests - 30 % of grade, written part of examination - 20 %, oral examination - 50 %;
Recommended literature
  • Holzbecher Z., Churáček J. a kol. Analytická chemie. Praha, 1987.
  • Kolektiv autorů. Instrumentální analýza. Praha, 1986.
  • Sommer L. Teoretické základy analytické chemie I, II, III. Brno, 1995.
  • Zýka J. (ed.). Analytická příručka, díl I a II. Praha, 1988.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Chemistry and Technical Chemistry (2015) Engineering chemistry and chemistry of silicates 3 Winter
Faculty of Chemical Technology Chemistry and Technical Chemistry (2014) Engineering chemistry and chemistry of silicates 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2015) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2013) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2016) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Chemistry and Technical Chemistry (2013) Engineering chemistry and chemistry of silicates 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2014) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Chemistry and Technical Chemistry (2016) Engineering chemistry and chemistry of silicates 3 Winter