Course: Laboratory of Clinical Biochemistry II

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Course title Laboratory of Clinical Biochemistry II
Course code KBBV/C041
Organizational form of instruction Lesson
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Královcová Pavla, Mgr. Ph.D.
  • Kanďár Roman, doc. Mgr. Ph.D.
Course content
Practice 1 Clinical Biochemistry laboratory: work safety Analytical parameters of method Concept definition: linearity, accuracy, precision, standard addition method, dilution experiment, robustness, limit of quantification, limit of detection Analytical parameters of selected method: intra-assay, linearity, limit of quantification and detection, accuracy of calibration curve (control serum), statistical evaluation (software Trilobyte, QCExpert), standard addition method, dilution experiment 8 hours Practice 2 Laboratory Information System LISSET: administration; LISANA: communication between LIS and analyzers, import of results Analyzers Cobas Mira and Olympus AU640, operation A comparison of results obtained by analyzers Olympus AU640, Cobas Mira (Roche) and nine-channel photometer Labsystems, statistical evaluation 8 hours Practice 3 The determination of alpha-tocopherol in plasma using high-performance liquid chromatography with coulometric electrochemical detection 8 hours Practice 4 Determination of selected antidepressants by gas chromatography with flame-ionization detector 8 hours

Learning activities and teaching methods
Laboratory work
  • unspecified - 40 hours per semester
  • unspecified - 30 hours per semester
Learning outcomes
The aim is to prepare the student for working in laboratories of Clinical Biochemistry.
Student will be able to work in laboratories of Clinical Biochemistry.
Prerequisites
KBBV/C346 or KBBV/C632 Laboratory of Clinical Biochemistry

Assessment methods and criteria
Written examination, Student performance assessment

Final written examination
Recommended literature
  • Doležalová V. a spol. Laboratorní technika v klinické biochemii a toxikologii, 1995, IVPZ Brno.
  • Doležalová, V. Principy biochemických vyšetřovacích metod. Část I. a II.. 1. vyd. IDVPZ, Brno 1995, 374 s. + 223 s.
  • Jaroslav Racek. Klinická biochemie. Galén, 2006. ISBN 80-7262-324-9.
  • Masopust, J. Klinická biochemie I, II, (požadování a hodnocení biochemických vyšetření), Karolinum, 1998.
  • Racek, J. a kol. Klinická biochemie, Galén, Karolinum, 1999.
  • Zima, T. Laboratorní diagnostika, Galén, Praha, 2002.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Analysis of Biological Materials (2016) Special and interdisciplinary fields 1 Summer
Faculty of Chemical Technology Bioanalyst (2016) Chemistry courses 1 Summer
Faculty of Chemical Technology Bioanalyst (2015) Chemistry courses 1 Summer
Faculty of Chemical Technology Analysis of Biological Materials (2015) Special and interdisciplinary fields 1 Summer
Faculty of Chemical Technology Bioanalyst (2013) Chemistry courses 1 Summer
Faculty of Chemical Technology Analysis of Biological Materials (2013) Special and interdisciplinary fields 1 Summer