Course: Laboratory of Microbiology I

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Course title Laboratory of Microbiology I
Course code KBBV/C348
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 2
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Moťková Petra, Ing. Ph.D.
  • Vydržalová Markéta, RNDr. Ph.D.
Course content
Safety and principles of work in microbiological laboratory, aseptic work, microbiological laboratory equipment. Methods of sterilization and disinfection. Basic terminology. Nutrient media (liquid and solid) - preparation and sterilization. Inoculation of microorganisms, practice of various inoculation techniques, isolation of pure culture, preservation and recovery of bacterial cultures. Cultivation of aerobic, anaerobic, microaerophilic microorganisms. Growth of microorganisms on solid and liquid nutrient media - determination of macroscopic characteristics. Physical, chemical and biological factors influencing the growth and reproduction of microorganisms - pH, temperature, UV radiation, osmotic pressure. Microscopic techniques (bright and dark field microscopy, fluorescence m., electron m.). Staining techniques - Gram staining. KOH test. Negative staining. Staining of spores and capsules. Acid-fast staining. Giemsa staining. Viruses - laboratory diagnostics. Cultivation of fungi important in the field of human microbiology - cultivation media, microscopic techniques. Biochemical tests based on carbohydrate transformation, transformation of nitrogen compounds. Other biochemical tests - production of hydrogen sulphide, production of catalase, oxidase, utilization of citrate. Commercial biochemical tests.

Learning activities and teaching methods
Laboratory work
Learning outcomes
Students are going to acquire the knowledge and skills to conduct various techniques used in laboratory of microbiology.
Students become acquainted with staining techniques, preparation of nutrient media and identification of microorganisms. Completion of the course is necessary for the further studies of special and clinical microbiology.
Prerequisites
KBBV/C604 - Optical microscopy techniques in health service

Assessment methods and criteria
Written examination

The knowledge is verified by written examination.
Recommended literature
  • VYTŘASOVÁ J., BÍLKOVÁ Z. Cvičení z obecné mikrobiologie. Univerzita Pardubice, 1998.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Laboratory Assistant (2013) Health service 2 Summer
Faculty of Chemical Technology Laboratory Assistant (2016) Health service 2 Summer
Faculty of Chemical Technology Laboratory Assistant (2014) Health service 2 Summer
Faculty of Chemical Technology Laboratory Assistant (2015) Health service 2 Summer