Course: Molecular Biologic Methods in Health Service

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Course title Molecular Biologic Methods in Health Service
Course code KBBV/C377
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Pejchalová Marcela, Ing. Ph.D.
  • Heidingsfeld Olga, RNDr. CSc.
Course content
Physical and chemical properties of nucleic acids (NA), their functions. Spectrofotometric determination of NA. Isolation of NA. Electrophoresis of nucleic acids. Replication, transcription, translation of NA. Polymerase chain reakctions. Gene, genotype, allels. Bacterial genome. Viral genome. Cloning of DNA. GMO.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
Learning outcomes
The objective is to acquaint students with structure, properties and functions of nucleic acids. A special attention is marked to genome of bacteria and viruses.Basic information about isolation of nucleic acids, real-time PCR, sequenation and hybridisation techniques, respectivelly. Disscused is also cloning of DNA,preparation and application og genetically modified organisms.
Students adopted basic information about methods of isolation of nucleic acids and molecular-biological methods (PCR, real-time PCR, RFLP, sequenation, hybrydization techniques), which are currently used in detection of inherited diseases.
Prerequisites
C501 Biology C607 Biochemistry

Assessment methods and criteria
Oral examination, Written examination

written or oral examination
Recommended literature
  • Dorian J. Pritchard, Bruce R. Korf. Základy lékařské genetiky. Praha, 2007. ISBN 978-80-7262-449-2.
  • Jan Kapras a kol. Kontrolní otázky Biologie a lékařská genetika. Praha, 1999. ISBN 80-7254-081-5.
  • Mazura I. Speciální metody molekulární biologie. Karolinum, Praha., 2001. ISBN 802460258.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Laboratory Assistant (2013) Health service 2 Summer
Faculty of Chemical Technology Laboratory Assistant (2016) Health service 2 Summer
Faculty of Chemical Technology Laboratory Assistant (2014) Health service 2 Summer
Faculty of Chemical Technology Laboratory Assistant (2015) Health service 2 Summer