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Course title -
Course code KBBV/C435
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Dvořáková Renata, RNDr.
  • Mrňáková Hana, Mgr.
  • Stříbrná Lucie, Mgr. Ph.D.
  • Pejchalová Marcela, doc. Ing. Ph.D.
Course content
1. Instruction to the safety at work. 2. Preparation and evaluation of prenatal diagnostic cytogenetic preparations (water, tissueof chorion). Evaluation findings, differential diagnosis, correlation with ultrasonic. 3. The preparation and evaluation of the postnatal diagnostic preparations (peripheral blood). Evaluation findings (use of molecular diagnostic differential). 4. Onkogenetics - cultivation and processing, bone marrow cells. Correlation of in the diagnosis, prognosis. Onkogenetics and monitoring treatment. 5. Cultivation and processing peripheral blood lymphocytes, obtained for chromosomal aberrations (ZCA). Cytogenetic methods in use. Chromosome instability syndromes. ¨ 6. Dyeing techniques and chromosome banding pattern, use in clinical practice. 7. Molecular cytogenetic methods: fluorescent in situ hybridization (FISH), arrayCGH, the latest trends. The use of differential diagnostic methods for the selection of the appropriate methodology. 8. The treatment of clinical genetics laboratory to clinical outcome and an anamnesis. 9. In cytogenetic laboratory quality control audits the internal quality control, quality control program. Model legislation and ethics, the situation in practice. 10. The isolation of NK theoretical introduction and practical role. 11. Electrophoresis NK theoretical introduction and separation of DNA. 12. Nobility.Examples of population genetics. H - W. law. 13. Model test. 14. Controling protocols, writing credits in the index of students.

Learning activities and teaching methods
Laboratory work
Learning outcomes
The main aim of this subject is to comprehend basic terms in genetics, inform about structure and function of nucleic acids and proteins. The main attention is paid to human genetics but also to genetic of bacteria and viruses. The others subjects are mutations and their types, sexual and asexual reproduction, cytogenetic and the most common genetic diseases in human population.
Students govern basic cytodiagnostic imaging techniques and basic methods used in molecular biology laboratories

Assessment methods and criteria
Home assignment evaluation

Credit is granted after all laboratory tasks, the development of appropriate protocols.
Recommended literature
  • KOČÁREK, Eduard, Martin PÁNEK a Drahuše NOVOTNÁ. Klinická cytogenetika I.: úvod do klinické cytogenetiky, vyšetřovací metody v klinické cytogenetice.. Praha, 2010. ISBN 80-246-1880-7.
  • KOTLAS, Jaroslav, et al. Návody a úkoly k praktickým cvičením z lékařské biologie a genetiky.. Praha, 2000. ISBN 80-246-0065-X.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Laboratory Assistant (2016) Health service 2 Winter
Faculty of Chemical Technology Laboratory Assistant (2015) Health service 2 Winter