Course: Laboratory of Microbiology

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Course title Laboratory of Microbiology
Course code KBBV/C509
Organizational form of instruction Lesson
Level of course Bachelor
Year of study 3
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Šilha David, Ing. Ph.D.
  • Pejchalová Marcela, Ing. Ph.D.
Course content
The principle of the work in microbiology lab, safety, equipment, desinfection, aseptic work, preparation of glassware. Microscopy. Native and simple staining, stable praparation. Diferentia and diagnostic staining of bacteria. Gram staining, negative staining. Staining of spores and capsules. Staining of volutine, proteins and nucelus in yeasts. Determination of the size of microorganisms. Preparation and effusion of nutrient bases. Inoculation, isolation of pure culture, storage and reviving of bacteria. The growth of microorganisms on the solid and liquid media. Identification of yeasts, blastospores and pseudomycelium. Microscopy of fungi, preparation of glass-slide culture. Determination of mobility of bacteria and the concentration of cells using Burker chamber. Substitute lab, written exam.

Learning activities and teaching methods
Laboratory work
Learning outcomes
The objective is to acquaint with the work´s principle and rules in microbiology lab, prepare various kind of microscopic preparations and nutrien media for growing of microorganisms. Students adopted various techniqes of inoculation and cultivation of various species and will be able to evaluate their characteristics.
Students govern a basic principle of microbiological work (aseptic), can prepare nutrient media and inoculate and cultivate various kind of microorganisms.
Prerequisites
KBBV/C501 and KBBV/C502
KBBV/C407
----- or -----
KBBV/C501 and KBBV/C535

Assessment methods and criteria
Written examination, Systematic monitoring

A credit is given upon the graduation of all the experiements, preparation of protocols and final check test.
Recommended literature
  • Šilhánková L., Demnerová K. Návody pro laboratoře z mikrobiologie. SNTL Praha, 1985..
  • Vytřasová J., Bílková Z. Návody pro laboratoře z mikrobiologie. Univerzita Pardubice, 1998..


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2015) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2013) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2016) Food industry and food industry chemistry 3 Winter
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2014) Food industry and food industry chemistry 3 Winter