Course: Laboratory of Microbiology II

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Course title Laboratory of Microbiology II
Course code KBBV/C516
Organizational form of instruction Lesson
Level of course Bachelor
Year of study 3
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Šilha David, Ing. Ph.D.
  • Pejchalová Marcela, doc. Ing. Ph.D.
Course content
Thermal inactivation, lethal temperature and time. The effect of pH, osmotic pressure and UV light. The effect of disinfection, antibiotics and various organic stains. The determination of bacterial count on the surfaces and in water samples. Biochemical test based on the saccharides metabolism. Biochemical test based on the nitrogen compounds metabolism. Other biochemical test. Diagnostic microtests, software TNW for identification of MO. Identification of yeasts (auxonograms, zymograms). Final examination work: identification of unknown samlpe containing two bacterial species. Final written exam.

Learning activities and teaching methods
Laboratory work
Learning outcomes
Practices of general microbiology, the influence of intrisic factors on the growth of microorganisms, the principles and preparation of biochemical tests for indentification of microorganisms. Students also govern determination of microorgnisms in air, water and surfaces.
Student governs kultivation techniques and identification of microorganisms using biochemical tests.
Prerequisites
KBBV/C509

Assessment methods and criteria
Written examination, Home assignment evaluation, Systematic monitoring

A classified credit is based on the assumption that student graduate all the experimental works, prepare protocols, final written exam and succesfull identification of test sample.
Recommended literature
  • Šilhánková L., Demnerová K. Návody pro laboratoře z mikrobiologie. SNTL Praha, 1985..
  • Vytřasová J., Bílková Z. Z.: Návody pro laboratoře z mikrobiologie. Univerzita Pardubice, 1998..


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2013) Food industry and food industry chemistry 3 Summer
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2014) Food industry and food industry chemistry 3 Summer
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2016) Food industry and food industry chemistry 3 Summer
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2015) Food industry and food industry chemistry 3 Summer