Course: Methods in Molecular Biology

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Course title Methods in Molecular Biology
Course code KBBV/C538
Organizational form of instruction Lecture
Level of course Bachelor
Year of study 3
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Pejchalová Marcela, Ing. Ph.D.
Course content
Physical and chemical properties of nucleic acids (NA), their functions. Spectrofotometric determination of NA. Isolation of NA. Electrophoresis of nucleic acids. Replication, transcription, translation of NA. Polymerase chain reakctions. Gene, genotype, allels. Bacterial genome. Viral genome. Cloning of DNA. GMO.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Demonstration
Learning outcomes
The objective is to acquaint students with structure, properties and functions of nucleic acids. A special attention is marked to genome of bacteria and viruses.
Students adopted basic information about methods of isolation of nucleic acids and molecular-biological methods (PCR, real-time PCR, RFLP, sequenation, hybrydization techniques), which are currently used in detection of pathogens.
Prerequisites
C 501 Biology

Assessment methods and criteria
Oral examination

essay and oral examination
Recommended literature
  • Clark D. P. Molecular Biology, 2005, Elsevier, Burlington..
  • Kočárek E. Genetika, 2004, Scientia, Praha..
  • Mazura I. Speciální metody molekulární biologie, 2001, Karolinum, Praha..
  • Rozsypal S. Úvod do molekulární biologie, 1996-2002, Brno..


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2013) Food industry and food industry chemistry 3 Summer
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2014) Food industry and food industry chemistry 3 Summer
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2016) Food industry and food industry chemistry 3 Summer
Faculty of Chemical Technology Evaluation and Analysis of Foodstuffs (2015) Food industry and food industry chemistry 3 Summer